transcriptional heterogeneity in naive and primed human

Patrick Wilson

Pauli NT Henry Dunand CJ Wilson PC Exploiting human memory B cell heterogeneity for improved vaccine efficacy Front Immunol 2011 2:77 View in: PubMed Smith K Crowe SR Garman L Guthridge CJ Muther JJ McKee E Zheng NY Farris AD Guthridge JM Wilson PC James JA Human monoclonal antibodies generated following vaccination with AVA

Acquisition of pluripotency in the chick embryo occurs

Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes amphibians and mammals have been elucidated

Fluidigm

Transcriptional Profiling of Human Familial Longevity Indicates a Role for ASF1A and IL7R Passtoors W M IL-2 imprints human naive B cell fate towards plasma cell through ERK/ELK1-mediated BACH2 repression Hipp N Symington H Pastoret C et al Nature Communications (2017): 1 443 PRR2 a pseudo-response regulator promotes salicylic acid and camalexin accumulation during plant

Culture adaptation alters transcriptional hierarchies

Culture adaptation alters transcriptional hierarchies among single human embryonic stem cells reflecting altered patterns of differentiation Authors: Paul J Gokhale Janice K Au-Young SriVidya Dadi David N Keys Neil J Harrison Mark Jones Shamit Soneji Tariq Enver Jon K Sherlock Peter W Andrews PLoS One 2015 14 10(4):e0123467 Epub 2015 Apr 14 Centre for Stem Cell Biology Department of

The mTOR pathway in reproduction: from gonadal function

At the same time primed cells heterogeneously upregulate the expression of lineage-commitment genes such as T-brachyury and Fgf5 (Tesar et al 2007 Guo et al 2009) Naive and primed cells differ not only in their developmental origin EpiSCs as mentioned can be derived from the post-implantation embryo and can also be obtained by the differentiation of mESC by culturing cells in the

Genome

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant pigs can result in congenital infection and ultimately fetal death Little is known about immune responses to infection at the maternal-fetal interface and in the fetus itself or the molecular events behind virus transmission and disease progression in the fetus To investigate these processes RNA-sequencing of

Intruders below the Radar: Molecular Pathogenesis of

Bartonella spp are facultative intracellular pathogens that employ a unique stealth infection strategy comprising immune evasion and modulation intimate interaction with nucleated cells and intraerythrocytic persistence Infections with Bartonella are ubiquitous among mammals and many species can infect humans either as their natural host or incidentally as zoonotic pathogens

Lung mucosal immunity: immunoglobulin

The molecular heterogeneity of IgA in serum and Naive B-lymphocytes enter through high endothelial venules by a multistep process of extravasation into inductive sites (reviewed in 20) There they are primed in extra-follicular areas by local CD4+ T-cells which are activated by interdigitating antigen-presenting cells (APCs) that have processed a luminal antigen 21 The surface (s) IgD +

Lars Steinmetz's Profile

Extensive transcriptional heterogeneity revealed by isoform profiling Nature Pelechano V Wei W Steinmetz L M 2013 497 (7447): 127-131 Abstract Transcript function is determined by sequence elements arranged on an individual RNA molecule Variation in transcripts can affect messenger RNA stability localization and translation or produce truncated proteins that differ in

Microglia response following acute demyelination is

Microglia and infiltrating macrophages are thought to orchestrate the central nervous system (CNS) response to injury however the similarities between these cells make it challenging to distinguish their relative contributions We genetically labeled microglia and CNS-associated macrophages to distinguish them from infiltrating macrophages Using single-cell RNA sequencing we describe

Transcriptional Heterogeneity in Naive and Primed Human

Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Tobias Messmer Cancer Research UK Cambridge Institute University of Cambridge Cambridge CB2 0RE UK Institute of Pharmacy and Molecular Biotechnology Heidelberg University Im Neuenheimer Feld 364 69120 Heidelberg Germany

Meet Jeffery

But leaving us to hypothesize that TERS could drive tumor clonal heterogeneity and provides tumor cell fitness 0:22:13 – Slide 20 To that end we used human prostate cancer cells as a model just so we could focus on one specific tissue The research being done today was predominantly using PC3 cells (inaudible) cells and DU145 cells In the first experiment that we performed we produced

Transcriptional Regulation of Innate and Adaptive

The lymphocyte family has expanded significantly in recent years to include not only the adaptive lymphocytes (T cells B cells) and NK cells but also several additional innate lymphoid cell (ILC) types ILCs lack clonally distributed antigen receptors characteristic of adaptive lymphocytes and instead respond exclusively to signaling via germline-encoded receptors ILCs resemble T cells more

Microglia Participate in Neurogenic Regulation of

In addition a group of mice were transferred with 110 5 Ang II–primed astrocytes Dashed line indicates the time point when ICV injection of Ang II C Quantification of magnitude and duration of blood pressure responses described in B *P0 05 vs nave †P0 01 vs naive microglia ‡P0 0001 vs Ang II–primed microglia by 1-way ANOVA

Mobile

Transfer of a copB-mco- carrying plasmid to a naive clinical isolate resulted in a gain of copper hypertolerance and enhanced bacterial survival inside primed macrophages The copB and mco genes were upregulated within infected macrophages and their expression was dependent on the copper-sensitive operon repressor CsoR Isogenic copB and mco mutants were impaired in their ability to

Ten years of progress and promise of induced pluripotent

The discovery of induced pluripotent stem cells (iPSCs) by Shinya Yamanaka in 2006 was heralded as a major breakthrough of the decade in stem cell research The ability to reprogram human somatic cells to a pluripotent embryonic stem cell-like state through the ectopic expression of a combination of embryonic transcription factors was greeted with great excitement by scientists and bioethicists

Zfp281 Coordinates Opposing Functions of Tet1 and Tet2

We identify Zfp281 as a key transcriptional regulator for primed pluripotency that also functions as a barrier toward achieving naive pluripotency in both mouse and human ESCs Mechanistically Zfp281 interacts with Tet1 but not Tet2 and its direct transcriptional target miR-302/367 to negatively regulate Tet2 expression to establish and maintain primed pluripotency Conversely ectopic

TeSR™

Several naive-specific but not primed-specific proteins were also expressed by pluripotent cells in the human preimplantation embryo The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations This analysis revealed distinct transcriptional and X chromosome

SETDB1 prevents TET2

Endogenous retroviruses (ERVs) which are responsible for 10% of spontaneous mouse mutations are kept under control via several epigenetic mechanisms The H3K9 histone methyltransferase SETDB1 is essential for ERV repression in embryonic stem cells (ESCs) with DNA methylation also playing an important role It has been suggested that SETDB1 protects ERVs from TET-dependent DNA

Cells

Expansion of hematopoietic stem cells (HSCs) for therapeutic purposes has been a "holy grail" in the field for many years Ex vivo expansion of HSCs can help to overcome material shortage for transplantation purposes and genetic modification protocols In this review we summarize improved understanding in blood development the effect of niche and conservative signaling pathways on HSCs

Mobile DNA

Heterogeneity of transposon expression and activation of the repressive network in human fetal germ cells [Development 2019] PubMed Retrophylogenomics in rorquals indicate large ancestral population sizes and a rapid radiation [Mob DNA 2019] PubMed Dynamic methylation of an L1 transduction family during reprogramming and neurodifferentiation

Transcriptional Heterogeneity in Naive and Primed Human

Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Author links open overlay panel Tobias Messmer 1 2 7 Ferdinand von Meyenn 3 4 7 8 Aurora Savino 3 Ftima Santos 3 Hisham Mohammed 3 9

Identification of novel HIV

The HIV-1 infection is characterized by profound CD4+ T cell destruction and a marked Th17 dysfunction at the mucosal level Viral suppressive antiretroviral therapy restores Th1 but not Th17 cells Although several key HIV dependency factors (HDF) were identified in the past years via genome-wide siRNA screens in cell lines molecular determinants of HIV permissiveness in primary Th17 cells

Recommended Treatment for Antibody

ment guidelines are based on low-level evidence The number of prospective randomized trials for the treatment of AMR is small and the lack of an accepted common standard for care has been an impediment to the development of new therapies To help alleviate this The Transplantation Society convened a meeting of international experts to develop a consensus as to what is appropriate

Culture adaptation alters transcriptional hierarchies

Culture adaptation alters transcriptional hierarchies among single human embryonic stem cells reflecting altered patterns of differentiation Authors: Paul J Gokhale Janice K Au-Young SriVidya Dadi David N Keys Neil J Harrison Mark Jones Shamit Soneji Tariq Enver Jon K Sherlock Peter W Andrews PLoS One 2015 14 10(4):e0123467 Epub 2015 Apr 14 Centre for Stem Cell Biology Department of

2019 Russell Ross Memorial Lecture in Vascular Biology

CD5 which distinguishes these subsets in mice does not differentiate human B-1 cells most CD5+ B cells are not CD20 + CD3 − CD27 + CD43 + B-1 cells and CD5 is not expressed on all B-1 cells 125 Studies from our laboratory also provide evidence that deeper heterogeneity exists in the human B-1 subset based on chemokine receptor expression 95 with expression of CXCR4 on circulating B-1

Consensus guidelines for the definition detection and

The majority of human tumors are not driven by active viral infections Nonetheless like pathogen-infected dying cells malignant cells can display a high antigenicity largely reflecting the elevated mutational rate that frequently accompanies malignant transformation and disease progression in the context of immunoevasion 96 97 In a fraction of cases such mutations prime immune responses

Epigenetic Control of Immunity

Immunity relies on the heterogeneity of immune cells and their ability to respond to pathogen challenges In the adaptive immune system lymphocytes display a highly diverse antigen receptor repertoire that matches the vast diversity of pathogens In the innate immune system the cell's heterogeneity and phenotypic plasticity enable flexible responses to changes in tissue homeostasis caused by

Introduction

This heterogeneity exists already at the monocyte level since monocytes can also belong to pro- or anti-inflammatory phenotypes Growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and M-CSF play a principal role in their activation: GM-CSF drives the differentiation of "pro-inflammatory" monocytes to M1 macrophages while M-CSF regulates differentiation of

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